Adhesion molecules in hematopoietic cells

Blood Cells. 1994;20(1):25-44.


Interaction with stromal cells is known to be crucial for growth and differentiation of hematopoietic cells. To characterize adhesion molecules involved in this interaction, we examined adhesion of a panel of lymphoid, myeloid, and mast cell lines with stromal cells. We found that very late antigen-4 (VLA-4) and vascular cell adhesion molecule 1 (VCAM-1) were major adhesion molecules in lymphoid and myeloid cells, whereas myeloma cells adhered to stromal cells through hyaluronate. We investigated regulation of VLA-4 during differentiation of myeloid cells using a neutrophil precursor cell line, L-G3. Differentiation of neutrophils induced by granulocyte colony-stimulating factor was accompanied with down-regulation of VLA-4. Induced L-G3 cells adhered to stromal cells in proportion to the expression of VLA-4. Mast cells used two mechanisms to adhere to fibroblasts and stromal cells. They adhered to fibronectin through VLA-5 when stimulated with steel factor and also directly to membrane-anchored steel factor through c-kit.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Adhesion / physiology
  • Cell Adhesion Molecules / blood*
  • Cell Adhesion Molecules / physiology
  • Cell Differentiation / physiology
  • Cell Line
  • Down-Regulation / physiology
  • Hematopoietic Cell Growth Factors / physiology
  • Hematopoietic Stem Cells / cytology*
  • Mice
  • Mice, Inbred BALB C
  • Neutrophils / cytology
  • Proto-Oncogene Proteins / physiology
  • Proto-Oncogene Proteins c-kit
  • Receptor Protein-Tyrosine Kinases / physiology
  • Receptors, Colony-Stimulating Factor / physiology
  • Receptors, Very Late Antigen / isolation & purification
  • Receptors, Very Late Antigen / metabolism
  • Stem Cell Factor
  • Stromal Cells / cytology
  • Tumor Cells, Cultured
  • Vascular Cell Adhesion Molecule-1


  • Cell Adhesion Molecules
  • Hematopoietic Cell Growth Factors
  • Proto-Oncogene Proteins
  • Receptors, Colony-Stimulating Factor
  • Receptors, Very Late Antigen
  • Stem Cell Factor
  • Vascular Cell Adhesion Molecule-1
  • Proto-Oncogene Proteins c-kit
  • Receptor Protein-Tyrosine Kinases