Abstract
Interaction with stromal cells is known to be crucial for growth and differentiation of hematopoietic cells. To characterize adhesion molecules involved in this interaction, we examined adhesion of a panel of lymphoid, myeloid, and mast cell lines with stromal cells. We found that very late antigen-4 (VLA-4) and vascular cell adhesion molecule 1 (VCAM-1) were major adhesion molecules in lymphoid and myeloid cells, whereas myeloma cells adhered to stromal cells through hyaluronate. We investigated regulation of VLA-4 during differentiation of myeloid cells using a neutrophil precursor cell line, L-G3. Differentiation of neutrophils induced by granulocyte colony-stimulating factor was accompanied with down-regulation of VLA-4. Induced L-G3 cells adhered to stromal cells in proportion to the expression of VLA-4. Mast cells used two mechanisms to adhere to fibroblasts and stromal cells. They adhered to fibronectin through VLA-5 when stimulated with steel factor and also directly to membrane-anchored steel factor through c-kit.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cell Adhesion / physiology
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Cell Adhesion Molecules / blood*
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Cell Adhesion Molecules / physiology
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Cell Differentiation / physiology
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Cell Line
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Down-Regulation / physiology
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Hematopoietic Cell Growth Factors / physiology
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Hematopoietic Stem Cells / cytology*
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Mice
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Mice, Inbred BALB C
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Neutrophils / cytology
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Proto-Oncogene Proteins / physiology
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Proto-Oncogene Proteins c-kit
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Receptor Protein-Tyrosine Kinases / physiology
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Receptors, Colony-Stimulating Factor / physiology
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Receptors, Very Late Antigen / isolation & purification
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Receptors, Very Late Antigen / metabolism
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Stem Cell Factor
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Stromal Cells / cytology
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Tumor Cells, Cultured
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Vascular Cell Adhesion Molecule-1
Substances
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Cell Adhesion Molecules
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Hematopoietic Cell Growth Factors
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Proto-Oncogene Proteins
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Receptors, Colony-Stimulating Factor
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Receptors, Very Late Antigen
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Stem Cell Factor
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Vascular Cell Adhesion Molecule-1
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Proto-Oncogene Proteins c-kit
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Receptor Protein-Tyrosine Kinases