Perlecan, basal lamina proteoglycan, promotes basic fibroblast growth factor-receptor binding, mitogenesis, and angiogenesis

Cell. 1994 Dec 16;79(6):1005-13. doi: 10.1016/0092-8674(94)90031-0.


A survey of defined species of cell surface and extracellular matrix heparan sulfate proteoglycans (HSPG) was performed in a search for cellular proteoglycans that can promote bFGF receptor binding and biological activity. Of the various affinity-purified HSPGs tested, perlecan, the large basement membrane HSPG, is found to induce high affinity binding of bFGF both to cells deficient in HS and to soluble FGF receptors. Heparin-dependent mitogenic activity of bFGF is strongly augmented by perlecan. Monoclonal antibodies to perlecan extract the receptor binding promoting activity from active HSPG preparations. In a rabbit ear model for in vivo angiogenesis, perlecan is a potent inducer of bFGF-mediated neovascularization. These results identify perlecan as a major candidate for a bFGF low affinity, accessory receptor and an angiogenic modulator.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Basement Membrane / chemistry
  • CHO Cells
  • Cell Division / drug effects
  • Cricetinae
  • Dose-Response Relationship, Drug
  • Ear / blood supply
  • Extracellular Matrix / metabolism
  • Female
  • Fibroblast Growth Factor 2 / metabolism*
  • Heparan Sulfate Proteoglycans*
  • Heparitin Sulfate / isolation & purification
  • Heparitin Sulfate / pharmacology*
  • Humans
  • Lung / chemistry
  • Neovascularization, Pathologic / metabolism*
  • Protein Binding
  • Proteoglycans / isolation & purification
  • Proteoglycans / pharmacology*
  • Rabbits
  • Receptors, Fibroblast Growth Factor / metabolism*


  • Heparan Sulfate Proteoglycans
  • Proteoglycans
  • Receptors, Fibroblast Growth Factor
  • Fibroblast Growth Factor 2
  • perlecan
  • Heparitin Sulfate