KIF1B, a novel microtubule plus end-directed monomeric motor protein for transport of mitochondria

Cell. 1994 Dec 30;79(7):1209-20. doi: 10.1016/0092-8674(94)90012-4.


To further elucidate the mechanism of organelle transport, we cloned a novel member of the mouse kinesin superfamily, KIF1B. This N-terminal-type motor protein is expressed ubiquitously in various kinds of tissues. In situ hybridization revealed that KIF1B is expressed abundantly in differentiated nerve cells. Interestingly, K1F1B works as a monomer, having a microtubule plus end-directed motility. Our rotary shadowing electron microscopy revealed mostly single globular structures. Immunocytochemically, KIF1B was colocalized with mitochondria in vivo. Furthermore, a subcellular fractionation study showed that KIF1B was concentrated in the mitochondrial fraction, and purified K1F1B could transport mitochondria along microtubules in vitro. These data strongly suggested that KIF1B works as a monomeric motor for anterograde transport of mitochondria.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Axonal Transport / physiology*
  • Biological Transport / physiology
  • Cells, Cultured
  • Cloning, Molecular
  • Gene Expression Regulation, Developmental
  • Kinesin / chemistry
  • Kinesin / genetics
  • Kinesin / physiology*
  • Mice
  • Microtubule-Associated Proteins / chemistry
  • Microtubule-Associated Proteins / genetics
  • Microtubule-Associated Proteins / physiology*
  • Mitochondria / physiology*
  • Molecular Sequence Data
  • Movement / physiology
  • Nerve Tissue Proteins / chemistry
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / physiology*
  • Protein Structure, Secondary
  • Rats
  • Recombinant Proteins / chemistry
  • Sequence Homology, Amino Acid
  • Tissue Distribution


  • Kif1b protein, mouse
  • Kif1b protein, rat
  • Microtubule-Associated Proteins
  • Nerve Tissue Proteins
  • Recombinant Proteins
  • Kinesin

Associated data

  • GENBANK/D17577