Cystic fibrosis transmembrane conductance regulator protein expression in brain

Neuroreport. 1994 Aug 15;5(13):1684-8. doi: 10.1097/00001756-199408150-00035.

Abstract

The cystic fibrosis transmembrane conductance regulator protein (CFTR) has been identified in bovine brain clathrin-coated vesicles, rat brain and a human neuroblastoma cell line using affinity-purified polyclonal peptide antibodies against CFTR. Immunocytochemical staining of multiple dendrites and soma of neurons of the diencephalon, midbrain, pons and medulla oblongata, has also been demonstrated. Whole cell lysates and membranes derived from rat brain, neuroblastoma cells and bovine brain clathrin-coated vesicles express the mature 150-165 kDa and 130 kDa unglycosylated forms of CFTR. The localization of CFTR to brain regions controlling homeostasis and energy expenditure may relate to the pathogenesis of non-pulmonary manifestations of cystic fibrosis. CFTR expression in neurons and coated vesicles suggests a possible effect on neuropeptide vesicle trafficking by mutant CFTR.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibody Specificity
  • Blotting, Western
  • Brain Chemistry / physiology*
  • Cattle
  • Cells, Cultured
  • Chloride Channels / metabolism*
  • Cystic Fibrosis / metabolism*
  • Cystic Fibrosis Transmembrane Conductance Regulator
  • Epitope Mapping
  • Humans
  • Immunohistochemistry
  • Membrane Proteins / biosynthesis*
  • Membrane Proteins / chemical synthesis
  • Molecular Sequence Data
  • Rats
  • Rats, Sprague-Dawley

Substances

  • CFTR protein, human
  • Chloride Channels
  • Membrane Proteins
  • Cystic Fibrosis Transmembrane Conductance Regulator