Univariate tolerance regions for fibrinogen, antithrombin III, protein C, protein S, plasminogen and alpha 2-antiplasmin in children using the new Automated Coagulation Laboratory (ACL) method

Klin Padiatr. Nov-Dec 1994;206(6):437-9. doi: 10.1055/s-2008-1046646.

Abstract

To avoid misclassification of lowered or enhanced coagulation proteins in childhood the purpose of this study was to establish functional normal ranges for healthy children aged 6 months to 16 years. PT, aPTT, fibrinogen, antithrombin III, protein C, protein S, plasminogen and alpha 2-antiplasmin were tested using the new Automated Coagulation Laboratory (ACL) method. Values for PT, aPTT, fibrinogen, antithrombin III, plasminogen and alpha 2-antiplasmin were closely comparable in all children although we found a minimum range of 61% in children aged 8 to 16 years in plasma concentrations of alpha 2-antiplasmin. Children < 2.5 years showed reduced lower boundaries encompassing 95% of the population for protein C and protein S activity, although medians for protein S activity were similar in all children. The rapid and automatic determination of functional coagulation proteins using chromogenic substrates on the ACL 300 (25-50 microliters citrated plasma/test) renders the possibility to realize a screening program for inherited thrombotic syndromes in a routine laboratory.

MeSH terms

  • Adolescent
  • Adult
  • Antithrombin III / analysis
  • Automation
  • Blood Coagulation / physiology*
  • Blood Coagulation Tests / methods*
  • Child
  • Child, Preschool
  • Female
  • Fibrinogen / analysis
  • Humans
  • Infant
  • Male
  • Plasminogen / analysis
  • Protein C / analysis
  • Protein S / analysis
  • Reference Values
  • alpha-2-Antiplasmin / analysis

Substances

  • Protein C
  • Protein S
  • alpha-2-Antiplasmin
  • Antithrombin III
  • Fibrinogen
  • Plasminogen