Expression of Mycoplasma Gallisepticum F-strain Surface Epitope

Avian Dis. Jul-Sep 1994;38(3):494-500.

Abstract

The expression of a Mycoplasma gallisepticum (MG) F-strain surface epitope was studied using monoclonal antibody 6F10. Different F strain colonies were cloned based on positive or negative expression of the 6F10-recognized epitope. Clones selected for the lack of expression of this epitope (negative clones) reverted rapidly to positive expression. On the other hand, negative clones derived from F-strain-vaccinated chickens expressed the 6F10-recognized epitope variably. After in vitro passages, two groups of cultures were observed. One group reverted rapidly to positive expression. The other group exhibited a very slow rate of reversion, with 30% or fewer of their colonies expressing the 6F10-recognized epitope. These results indicate that the 6F10-recognized surface epitope of F-strain is variably expressed under in vivo and in vitro conditions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Antigens, Bacterial / metabolism*
  • Antigens, Surface / metabolism
  • Bacterial Vaccines / pharmacology
  • Chickens / immunology*
  • Chickens / microbiology*
  • Epitopes / metabolism
  • Mycoplasma / classification
  • Mycoplasma / growth & development
  • Mycoplasma / immunology*
  • Mycoplasma Infections / prevention & control
  • Mycoplasma Infections / veterinary
  • Poultry Diseases / prevention & control

Substances

  • Antibodies, Monoclonal
  • Antigens, Bacterial
  • Antigens, Surface
  • Bacterial Vaccines
  • Epitopes