Induction of HLA-DR antigen expression on human colonic epithelium by tumor necrosis factor-alpha and interferon-gamma

Scand J Gastroenterol. 1994 Oct;29(10):903-7. doi: 10.3109/00365529409094861.

Abstract

Background: We investigated the effects of tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) on HLA-DR expression on human normal colonic epithelium, using an organ culture technique.

Methods: Biopsy specimens from normal colonic mucosa were cultured for 24 h with various concentrations of TNF-alpha and/or IFN-gamma. Epithelial HLA-DR antigens were identified by an indirect immunoperoxidase staining method.

Results: No expression of epithelial HLA-DR antigens was observed in specimens cultured with medium alone. Concentrations of 10(3) U/ml or higher of TNF-alpha and 10(2) U/ml or higher of IFN-gamma induced expression of HLA-DR antigens in a dose-dependent manner. Expression of HLA-DR was increased in specimens cultured with both TNF-alpha and IFN-gamma compared with those cultured with either cytokine alone.

Conclusions: Our findings suggest that both TNF-alpha and IFN-gamma play important roles in regulating the expression of HLA-DR on colonic epithelium in patients with inflammatory bowel disease.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Colon, Sigmoid / drug effects
  • Colon, Sigmoid / metabolism*
  • Culture Media
  • Dose-Response Relationship, Drug
  • Epithelium / drug effects
  • Epithelium / metabolism
  • Female
  • HLA-DR Antigens / biosynthesis*
  • HLA-DR Antigens / drug effects
  • Humans
  • Interferon-gamma / pharmacology*
  • Intestinal Mucosa / drug effects
  • Intestinal Mucosa / metabolism*
  • Male
  • Organ Culture Techniques
  • Rectum / drug effects
  • Rectum / metabolism*
  • Staining and Labeling
  • Tumor Necrosis Factor-alpha / pharmacology*

Substances

  • Culture Media
  • HLA-DR Antigens
  • Tumor Necrosis Factor-alpha
  • Interferon-gamma