Characterization of two Epstein-Barr virus epitopes restricted by HLA-B7

Eur J Immunol. 1995 Jan;25(1):18-24. doi: 10.1002/eji.1830250105.


We have identified two epitopes for Epstein-Barr virus specific cytotoxic T lymphocytes (CTL) restricted by the common allele HLA-B7. They are EBNA 3C 881-9 (QPRAPIRPI) and EBNA 3A 379-387 (RPPIFIRRL). The epitopes conform well to the recently described motif for HLA-B7-binding peptides (Huckzo et al., J. Immunol. 1993. 151:2572). Titration of the peptides in CTL assays and detergent lysate binding assays revealed that extending the peptides at either the N or C terminus did not reduce their affinity for HLA-B7. This behavior contrasted with HLA-B51, which binds peptides with a similar motif to B7, and has identical amino acid residues at sites expected to form the "F" pocket of the peptide-binding groove. HLA-B51 also bound the peptide EBNA 3C 881-9, but was unable to bind peptides extended at the C terminus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antigens, Viral / immunology*
  • Cell Line
  • Clone Cells
  • Cytotoxicity Tests, Immunologic
  • DNA-Binding Proteins / immunology*
  • Epitopes / immunology
  • Epstein-Barr Virus Nuclear Antigens
  • HLA-B7 Antigen / genetics*
  • HLA-B7 Antigen / immunology
  • Herpesvirus 4, Human / immunology*
  • Humans
  • Molecular Sequence Data
  • Peptide Fragments / chemical synthesis
  • Peptide Fragments / immunology
  • Protein Binding / immunology
  • T-Lymphocytes, Cytotoxic / immunology
  • Transfection


  • Antigens, Viral
  • DNA-Binding Proteins
  • Epitopes
  • Epstein-Barr Virus Nuclear Antigens
  • HLA-B7 Antigen
  • Peptide Fragments