The jellyfish green fluorescent protein: a new tool for studying ion channel expression and function

Neuron. 1995 Feb;14(2):211-5. doi: 10.1016/0896-6273(95)90279-1.


Two methods are described for using the jellyfish green fluorescent protein (GFP) as a reporter gene for ion channel expression. GFP fluorescence can be used to identify the transfected cells, and to estimate the relative levels of ion channel expression, in cotransfection experiments. A GFP-NMDAR1 chimera can be constructed that produces a functional, fluorescent receptor subunit. These methods should facilitate studies of ion channel expression, localization, and processing.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Line
  • Evoked Potentials
  • Green Fluorescent Proteins
  • Humans
  • Ion Channel Gating
  • Ion Channels / biosynthesis*
  • Ion Channels / physiology
  • Kidney
  • Luminescent Proteins / analysis
  • Luminescent Proteins / biosynthesis*
  • Macromolecular Substances
  • Microscopy, Fluorescence / methods
  • Patch-Clamp Techniques
  • Polymerase Chain Reaction
  • Receptors, N-Methyl-D-Aspartate / biosynthesis*
  • Receptors, N-Methyl-D-Aspartate / physiology
  • Recombinant Fusion Proteins / biosynthesis
  • Scyphozoa
  • Transfection / methods


  • Ion Channels
  • Luminescent Proteins
  • Macromolecular Substances
  • Receptors, N-Methyl-D-Aspartate
  • Recombinant Fusion Proteins
  • Green Fluorescent Proteins