Protein (western) blotting

Mol Biotechnol. 1994 Jun;1(3):289-305. doi: 10.1007/BF02921696.

Abstract

The different steps involved in protein (Western) blotting and subsequent analysis of the proteins are reviewed. Electrophoretic separation of proteins, procedures of transfer to membranes, immunological and nonimmunological protein detection systems, and characterization of protein-nucleic acid and protein-protein interactions are described. Emphasis is on the sensitivity of the methods described and on possible variations that allow the individual steps of Western blotting to be adapted to specific questions.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Antibodies
  • Biotechnology
  • Blotting, Western / methods*
  • Blotting, Western / statistics & numerical data
  • Buffers
  • DNA-Binding Proteins / isolation & purification
  • Electrophoresis, Polyacrylamide Gel / methods
  • Membranes, Artificial
  • Proteins / immunology
  • Proteins / isolation & purification*
  • RNA-Binding Proteins / isolation & purification
  • Sensitivity and Specificity
  • Staining and Labeling / methods

Substances

  • Antibodies
  • Buffers
  • DNA-Binding Proteins
  • Membranes, Artificial
  • Proteins
  • RNA-Binding Proteins