Sialoadhesin binds preferentially to cells of the granulocytic lineage

J Clin Invest. 1995 Feb;95(2):635-43. doi: 10.1172/JCI117708.

Abstract

Sialoadhesin is a macrophage-restricted, sialic acid-dependent receptor of 185 kD that binds to the oligosaccharide sequence NeuAc alpha 2,3Gal on cell surface glycoconjugates. Recent cDNA cloning has shown that sialoadhesin is a new member of the immunoglobulin superfamily with sequence similarity to CD22, a sialic acid-dependent receptor of B lymphocytes. Sialoadhesin has been implicated in cellular interactions of stromal macrophages with developing myeloid cells. In this study, direct evidence for this interaction was obtained in cell-cell binding assays using both native and recombinant forms of the protein. In all assays, sialoadhesin exhibited specific, differential binding to various murine cell populations of hemopoietic origin. In rank order, sialoadhesin bound neutrophils > bone marrow cells = blood leukocytes > lymphocytes > thymocytes. Single-cell analyses confirmed that sialoadhesin selectively bound myeloid cells in complex cell mixtures obtained from the bone marrow and blood. In comparison, a recombinant Fc-chimeric form of murine CD22 showed high binding to B and T lymphocytes, but very low binding to immature and mature myeloid cells. These results are consistent with the notion that sialoadhesin in involved in interactions with granulocytes at different stages of their life histories.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Cell Adhesion Molecules / immunology
  • Cell Adhesion Molecules / metabolism
  • Cell Line
  • Chlorocebus aethiops
  • Cloning, Molecular
  • DNA, Complementary
  • Epitopes / analysis
  • Erythrocytes / immunology*
  • Erythrocytes / metabolism
  • Female
  • Glycoconjugates / chemistry
  • Glycoconjugates / immunology
  • Glycoconjugates / metabolism
  • Granulocytes / immunology*
  • Granulocytes / metabolism
  • Immunoglobulin Fab Fragments / pharmacology
  • Immunoglobulin G / pharmacology
  • Kinetics
  • Male
  • Membrane Glycoproteins / immunology
  • Membrane Glycoproteins / isolation & purification
  • Membrane Glycoproteins / metabolism*
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Receptors, Immunologic / immunology
  • Receptors, Immunologic / isolation & purification
  • Receptors, Immunologic / metabolism*
  • Recombinant Proteins / immunology
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • Sialic Acid Binding Ig-like Lectin 1
  • Transfection

Substances

  • Cell Adhesion Molecules
  • DNA, Complementary
  • Epitopes
  • Glycoconjugates
  • Immunoglobulin Fab Fragments
  • Immunoglobulin G
  • Membrane Glycoproteins
  • Receptors, Immunologic
  • Recombinant Proteins
  • Sialic Acid Binding Ig-like Lectin 1
  • Siglec1 protein, mouse