Lipofection of synthetic oligodeoxyribonucleotide having a palindromic sequence of AACGTT to murine splenocytes enhances interferon production and natural killer activity

Microbiol Immunol. 1994;38(10):831-6. doi: 10.1111/j.1348-0421.1994.tb01867.x.


A synthetic 22-mer oligodeoxyribonucleotide having an AACGTT palindrome, AAC-22, induced interferon (IFN) production and augmented the natural killer (NK) activity in murine splenocytes, whereas its analogue, ACC-22, having an ACCGGT palindrome, did not. The binding of AAC-22 to splenocytes was not different from that of ACC-22. Lipofection of AAC-22 to splenocytes remarkably enhanced IFN production and NK cell activity, whereas that of ACC-22 caused little enhancement. These results strongly suggest that the prerequisite for IFN production is not the binding of AAC-22 to the cell surface receptors, but its penetration into the spleen cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cells, Cultured
  • DNA / immunology*
  • Female
  • Interferons / biosynthesis*
  • Killer Cells, Natural / immunology*
  • Liposomes
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides / chemical synthesis
  • Oligodeoxyribonucleotides / immunology*
  • Spleen / immunology*


  • Liposomes
  • Oligodeoxyribonucleotides
  • DNA
  • Interferons