Characterization of a LINE-1 cDNA that originated from RNA present in ribonucleoprotein particles: implications for the structure of an active mouse LINE-1

Gene. 1995 Feb 14;153(2):261-6. doi: 10.1016/0378-1119(94)00785-q.

Abstract

Full-length, sense-strand, long interspersed element-1 (LINE-1 or L1) RNA is found as an RNA-protein complex in mouse embryonal carcinoma cells. Since this complex is a likely intermediate in LINE-1 transposition, its RNA may be enriched for the functional, or active, subset of mouse L1 sequences. For this reason, a cDNA library was constructed from RNA prepared from these ribonucleoprotein particles. The isolation and complete DNA sequence of one clone that is a strong candidate to be a functional version of mouse L1 is reported here. The structure of this element suggests a revision of the predicted sequence of an active mouse L1 and provides a tag that can be used to isolate its locus in the genome.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Carcinoma, Embryonal
  • Cloning, Molecular
  • DNA, Complementary / genetics*
  • Genetic Variation / genetics
  • Mice
  • Molecular Sequence Data
  • Open Reading Frames / genetics
  • RNA / genetics*
  • Repetitive Sequences, Nucleic Acid / genetics*
  • Retroelements / genetics*
  • Ribonucleoproteins / chemistry
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid

Substances

  • DNA, Complementary
  • Retroelements
  • Ribonucleoproteins
  • RNA

Associated data

  • GENBANK/U15647