Effect of tumor necrosis factor-alpha on a cell line transformed by a secreted form of human fibroblast growth factor-1 gene and on its parental cell line

Cancer Lett. 1995 Feb 10;89(1):49-54. doi: 10.1016/0304-3835(95)90157-4.


In the present study we characterized the responses of the cell line HST-alpha, stably transformed by a secreted form of human fibroblast growth factor-1 (acidic FGF) gene, and its parental NIH 3T3 cell line to recombinant murine (rMu) TNF-alpha. Treatment of HST-alpha cells with rMu TNF-alpha can significantly reduce the number of foci formed in the in vitro transformation assay. In the presence of the RNA polymerase inhibitor actinomycin-D, the transformed HST-alpha cell is more susceptible to the cytotoxicity of rMu TNF-alpha than is its parental NIH 3T3 cell. The median lethal concentrations (LC50) of rMu TNF-alpha to HST-alpha cell and NIH 3T3 cell were 0.35 ng/ml and 4.56 ng/ml respectively. These results demonstrated that the introduction of a single oncogene or a growth factor gene to a cell can change the cell's response to cytokines.

Publication types

  • Comparative Study

MeSH terms

  • 3T3 Cells / drug effects*
  • 3T3 Cells / physiology*
  • Animals
  • Cell Line, Transformed / drug effects
  • Fibroblast Growth Factor 1 / genetics*
  • Fibroblast Growth Factor 1 / metabolism*
  • Humans
  • Mice
  • Recombinant Proteins / pharmacology
  • Transformation, Genetic*
  • Tumor Necrosis Factor-alpha / pharmacology*


  • Recombinant Proteins
  • Tumor Necrosis Factor-alpha
  • Fibroblast Growth Factor 1