Up-regulation of nitric oxide synthase by estradiol in human aortic endothelial cells

FEBS Lett. 1995 Mar 6;360(3):291-3. doi: 10.1016/0014-5793(95)00124-r.


We have examined the effects of sex hormones on calcium-dependent NO production and protein levels of NO synthase in cultured human aortic endothelial cells, which were treated with various doses of 17 beta-estradiol and testosterone for 8-48 h. Treatment with 17 beta-estradiol enhanced calcium-dependent NO production, but testosterone had exerted no effect. Western blot using monoclonal anti-human endothelial NO synthase antibody clarified that increased NO production by 17 beta-estradiol treatment was accompanied by increased NO synthase protein. Our results provide evidence that human endothelial NO synthase can be regulated by estrogens.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Oxidoreductases / metabolism*
  • Aorta
  • Base Sequence
  • Cells, Cultured
  • Endothelium, Vascular / enzymology*
  • Enzyme Induction / drug effects
  • Estradiol / pharmacology*
  • Gene Expression Regulation, Enzymologic / drug effects
  • Humans
  • In Vitro Techniques
  • Molecular Sequence Data
  • Nitric Oxide Synthase
  • Regulatory Sequences, Nucleic Acid
  • Tamoxifen / pharmacology
  • Testosterone / pharmacology


  • Tamoxifen
  • Testosterone
  • Estradiol
  • Nitric Oxide Synthase
  • Amino Acid Oxidoreductases