[Intensification of sensitivity of monoclonality determination of B-cells in autoimmune thyroiditis and low malignancy B-NHL of the MALT type using temperature gradient gel electrophoresis (TGGE)]

Verh Dtsch Ges Pathol. 1994:78:208-13.
[Article in German]


Malignant non-Hodgkins lymphoma with primary involvement of the thyroid is a rare disease. Nearly all these lymphomas are of B-cell phenotype, and they represent a broad morphological spectrum of high and low grade entities. We investigated 18 cases of Hashimotos disease and 95 cases of thyroid non-Hodgkins lymphoma including 16 cases of MALT-lymphoma. This type of lymphoma is often hardly distinguishable from reactive lesions, even by immunohistochemistry. Therefore, we introduced a new molecular genetic technique based on the polymerase chain reaction (PCR) combined with temperature-gradient gel electrophoresis to demonstrate monoclonal populations of B cells in a polyclonal background of reactive B lymphocytes. With this approach we found monoclonality in 4 of 18 cases of Hashimotos disease. In our opinion, these 4 cases demonstrate clearly the transition from autoimmune disease into non-Hodgkins lymphoma.

Publication types

  • English Abstract

MeSH terms

  • B-Lymphocytes / immunology*
  • Clonal Anergy
  • DNA / analysis
  • Electrophoresis / methods
  • Gene Rearrangement
  • Genes, Immunoglobulin
  • Humans
  • Lymphoma, B-Cell, Marginal Zone / genetics
  • Lymphoma, B-Cell, Marginal Zone / immunology*
  • Lymphoma, B-Cell, Marginal Zone / pathology
  • Polymerase Chain Reaction / methods
  • Receptors, Antigen, T-Cell / genetics
  • Sensitivity and Specificity
  • Temperature
  • Thyroiditis, Autoimmune / genetics
  • Thyroiditis, Autoimmune / immunology*
  • Thyroiditis, Autoimmune / pathology


  • Receptors, Antigen, T-Cell
  • DNA