Fluorescence resonance energy transfer

Curr Opin Biotechnol. 1995 Feb;6(1):103-10. doi: 10.1016/0958-1669(95)80016-6.

Abstract

In the past year, a number of studies have demonstrated the utility of fluorescence resonance energy transfer as a technique for probing complex intermolecular interactions and for determining the spatial extension and geometrical characteristics of multicomponent structures composed of diverse molecular constituents, such as proteins, lipids, carbohydrates, nucleic acids, and even cells with viruses. The benefits of fluorescence resonance energy transfer are becoming increasingly evident to researchers who require measurements with high sensitivity, specificity, non-invasiveness, rapidity, and relative simplicity.

Publication types

  • Review

MeSH terms

  • Animals
  • Carbohydrates / chemistry*
  • DNA / chemistry*
  • DNA / metabolism
  • Energy Transfer
  • Humans
  • Lipids / chemistry
  • Proteins / chemistry*
  • Proteins / metabolism
  • RNA / chemistry*
  • RNA / metabolism
  • Spectrometry, Fluorescence / methods*
  • Virus Physiological Phenomena

Substances

  • Carbohydrates
  • Lipids
  • Proteins
  • RNA
  • DNA