Human B7-1 (CD80) and B7-2 (CD86) bind with similar avidities but distinct kinetics to CD28 and CTLA-4 receptors

Immunity. 1994 Dec;1(9):793-801. doi: 10.1016/s1074-7613(94)80021-9.


B7-0 or B7-2 (CD86) is a T cell costimulatory molecule that binds the same receptors (CD28 and CTLA-4) as B7-1 (CD80), but shares with it only approximately 25% sequence identity and is expressed earlier during an immune response. Here we show that human CD86 maintains similar (within approximately 2- to 3-fold) overall receptor binding and T cell costimulatory properties as CD80. However, CD80 and CD86 did not bind equivalently to CTLA-4: CD80 bound Y100A, a form of CTLA4lg with a mutation in the CDR3-like region, > 200-fold better than did CD86; inhibition of CD80-mediated cellular responses required approximately 100-fold lower CTLA4lg concentrations; and CD80-CTLA4lg complexes dissociated 5- to 8-fold more slowly, Thus, CD80 and CD86 utilize different binding determinants and have different kinetics of binding to CD28 and CTLA-4.

Publication types

  • Comparative Study

MeSH terms

  • Abatacept
  • Animals
  • Antigens, CD*
  • Antigens, Differentiation / chemistry
  • Antigens, Differentiation / genetics
  • Antigens, Differentiation / metabolism*
  • B7-1 Antigen / chemistry
  • B7-1 Antigen / metabolism*
  • B7-2 Antigen
  • Base Sequence
  • Binding Sites
  • CD28 Antigens / chemistry
  • CD28 Antigens / metabolism*
  • CHO Cells
  • CTLA-4 Antigen
  • Cricetinae
  • DNA, Complementary
  • Humans
  • Immunoconjugates*
  • Kinetics
  • Membrane Glycoproteins / chemistry
  • Membrane Glycoproteins / metabolism*
  • Molecular Sequence Data
  • Recombinant Fusion Proteins / metabolism
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / metabolism


  • Antigens, CD
  • Antigens, Differentiation
  • B7-1 Antigen
  • B7-2 Antigen
  • CD28 Antigens
  • CD86 protein, human
  • CTLA-4 Antigen
  • CTLA4 protein, human
  • DNA, Complementary
  • Immunoconjugates
  • Membrane Glycoproteins
  • Recombinant Fusion Proteins
  • Abatacept