Erythropoietin induces tyrosine phosphorylation of the beta chain of the GM-CSF receptor

Biochem Biophys Res Commun. 1995 Mar 28;208(3):1060-6. doi: 10.1006/bbrc.1995.1442.


Granulocyte-macrophage colony-stimulating factor (GM-CSF) and erythropoietin (EPO) induce tyrosine phosphorylation of the GM-CSF receptor beta chain and the EPO receptor, respectively, although their receptors lack the tyrosine kinase activity. We have shown that EPO as well as GM-CSF induces tyrosine phosphorylation of the beta chain. Conversely, GM-CSF does not induce tyrosine phosphorylation of the EPO receptor. Tyrosine phosphorylation of the beta chain by stimulation with EPO is rapid and transient. EPO may trans-modulate a signaling pathway of GM-CSF by phosphorylating the beta chain of the GM-CSF receptor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Cell Line
  • Electrophoresis, Polyacrylamide Gel
  • Erythropoietin / pharmacology*
  • Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology
  • Humans
  • Immunoblotting
  • Macromolecular Substances
  • Molecular Sequence Data
  • Phosphoproteins / isolation & purification
  • Phosphoproteins / metabolism
  • Phosphorylation
  • Phosphotyrosine
  • Receptors, Erythropoietin / metabolism
  • Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / isolation & purification
  • Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / metabolism*
  • Recombinant Proteins / pharmacology
  • Tyrosine / analogs & derivatives*
  • Tyrosine / analysis
  • Tyrosine / metabolism


  • Macromolecular Substances
  • Phosphoproteins
  • Receptors, Erythropoietin
  • Receptors, Granulocyte-Macrophage Colony-Stimulating Factor
  • Recombinant Proteins
  • Erythropoietin
  • Phosphotyrosine
  • Tyrosine
  • Granulocyte-Macrophage Colony-Stimulating Factor