CD8+ and CD45RA+ human peripheral blood lymphocytes are potent sources of macrophage inflammatory protein 1 alpha, interleukin-8 and RANTES

Eur J Immunol. 1995 Mar;25(3):751-6. doi: 10.1002/eji.1830250319.


The chemokines macrophage inflammatory protein 1 alpha (MIP 1 alpha), interleukin-8 (IL-8) and RANTES are potent regulators of leukocyte trafficking. Examination of chemokine secretion by human peripheral blood lymphocytes after stimulation with anti-CD3 or phorbol 12, 13 myristate acetate and ionomycin showed CD8+ cells were the dominant source of MIP 1 alpha and RANTES. Although production of MIP 1 alpha and IL-8 were similar in pharmacologically stimulated CD4+ CD45RA+, CD4+ CD45RO+, and CD8+ CD45RA+ cells, the largest amounts of MIP 1 alpha and RANTES were secreted by CD8+ CD45RO+ lymphocytes. A parallel pattern of prolonged chemokine mRNA expression for at least 18 h after activation was observed in the T cells subsets. These results confirm that human T lymphocytes have a unique capacity for secretion of these three chemokines. In addition, CD8+ cells have an unrecognized role in recruiting cells to sites of inflammation, and adult human CD45RA+ cells have a physiologically significant secretory capacity.

MeSH terms

  • Blotting, Northern
  • CD4-Positive T-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes / immunology
  • Chemokine CCL3
  • Chemokine CCL4
  • Chemokine CCL5
  • Cytokines / biosynthesis*
  • Flow Cytometry
  • Humans
  • Interleukin-8 / biosynthesis
  • Leukocyte Common Antigens / immunology
  • Lymphocyte Activation / immunology
  • Lymphokines / biosynthesis
  • Macrophage Inflammatory Proteins
  • Monokines / biosynthesis
  • RNA, Messenger / biosynthesis
  • T-Lymphocyte Subsets / immunology*


  • Chemokine CCL3
  • Chemokine CCL4
  • Chemokine CCL5
  • Cytokines
  • Interleukin-8
  • Lymphokines
  • Macrophage Inflammatory Proteins
  • Monokines
  • RNA, Messenger
  • Leukocyte Common Antigens