Functional characterization of rat chemokine macrophage inflammatory protein-2

Inflammation. 1995 Feb;19(1):133-42. doi: 10.1007/BF01534386.

Abstract

Expression of mRNA for the C-X-C chemokine, macrophage inflammatory protein-2 (MIP-2), is induced during acute inflammation in rat models of disease. We have characterized the phlogistic potential of rat recombinant MIP-2 (rMIP-2) protein in vitro and in vivo. Recombinant MIP-2 caused marked PMN chemotaxis in vitro, with peak chemotactic activity at 10 nM. Incubation of whole blood with rMIP-2 caused a significant loss of L-selectin and a significant increase in Mac-1 expression on the PMN surface. Under similar conditions rMIP-2 also caused a modest respiratory burst in PMNs. The intratracheal instillation of 10 and 50 micrograms of rMIP-2 caused a significant influx of PMNs into the airspace of the lungs. Rat MIP-2 is a potent neutrophil chemotactic factor capable of causing neutrophil activation and is likely to function in PMN recruitment during acute inflammation in rat disease models.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Cell Adhesion Molecules / metabolism
  • Cell Movement / physiology
  • Cells, Cultured
  • Chemokine CXCL2
  • Chemotactic Factors / physiology
  • Cytokines / physiology*
  • Electrophoresis, Polyacrylamide Gel
  • L-Selectin
  • Macrophage-1 Antigen / metabolism
  • Membrane Glycoproteins / metabolism
  • Molecular Sequence Data
  • Monokines / physiology*
  • Neutrophils / physiology
  • Oligonucleotide Probes / genetics
  • Rats
  • Respiratory Burst

Substances

  • Cell Adhesion Molecules
  • Chemokine CXCL2
  • Chemotactic Factors
  • Cytokines
  • Macrophage-1 Antigen
  • Membrane Glycoproteins
  • Monokines
  • Oligonucleotide Probes
  • L-Selectin