Prevention of in vitro neutrophil-endothelial attachment through shedding of L-selectin by nonsteroidal antiinflammatory drugs

J Clin Invest. 1995 Apr;95(4):1756-65. doi: 10.1172/JCI117853.


The activation of the endothelial cells by extravascular stimuli is the key event in the extravasation of circulating leukocytes to target tissues. L-selectin, a member of the selectin family, is constitutively expressed by white cells, and is the molecule involved in the initial binding of leukocytes to activated endothelium. After activation, leukocytes rapidly release L-selectin from the cell surface, suggesting that the functional activity of this molecule is controlled in large part by its appearance and disappearance from cell surface. We have studied in a neutrophil-activated endothelial cell binding assay, the effect of different antiinflammatory drugs (steroidal and nonsteroidal) in the L-selectin-mediated interaction of neutrophils with activated endothelial cells. Some nonsteroidal antiinflammatory drugs (NSAIDs), such as indomethacin, diclofenac, ketoprofen, and aspirin, but not steroids, strongly inhibited the neutrophil-endothelial cell attachment. Furthermore, we also investigated the underlying mechanism of this functional effect. The expression of L-selectin on the neutrophil surface rapidly decreased in the presence of different NSAIDs, in a dose- and time-dependent manner, whereas no changes in the expression of other adhesion molecules such as CD11a, CD11b, CD31, or ICAM-3 (CD50) were observed. Interestingly, studies in vivo on healthy volunteers treated with physiological doses of indomethacin showed a significant decrease of L-selectin neutrophil expression. Only diclofenac induced an upregulation of CD11b expression, suggesting an activating effect on neutrophils. No enzyme release was observed upon treatment of neutrophils with different NSAIDs, indicating a lack of degranulatory activity of NSAIDs, with the exception of diclofenac. The downregulation of L-selectin expression was due to the rapid cleavage and shedding of the membrane L-selectin, as determined by both immunoprecipitation from 125I-labeled neutrophils, and quantitative estimation in cell-free supernatants. These results suggest that NSAIDs exert a specific action on adhesion receptor expression in neutrophils, which might account, at least in part, for the antiinflammatory activities of NSAIDs.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
  • Antigens, CD / biosynthesis
  • Aspirin / pharmacology
  • Cell Adhesion / drug effects*
  • Cell Adhesion Molecules / metabolism*
  • Cytoplasmic Granules / enzymology
  • Diclofenac / pharmacology
  • Dose-Response Relationship, Drug
  • Down-Regulation
  • Endothelium, Vascular / physiology*
  • Enzymes / metabolism
  • Humans
  • Indomethacin / pharmacology
  • Inflammation / etiology
  • Intercellular Adhesion Molecule-1 / biosynthesis
  • L-Selectin
  • Neutrophils / physiology*
  • Piroxicam / pharmacology


  • Anti-Inflammatory Agents, Non-Steroidal
  • Antigens, CD
  • Cell Adhesion Molecules
  • Enzymes
  • Intercellular Adhesion Molecule-1
  • L-Selectin
  • Piroxicam
  • Diclofenac
  • Aspirin
  • Indomethacin