Detection of Helicobacter pylori infection in early stage gastric cancer. A comparison between intestinal- and diffuse-type gastric adenocarcinomas

Cancer. 1995 May 1;75(9):2203-8. doi: 10.1002/1097-0142(19950501)75:9<2203::aid-cncr2820750903>;2-6.


Background: Helicobacter pylori (H pylori) infection has been suggested to be a risk factor for gastric carcinogenesis. However, those previous studies have been concerned with advanced cancer cases. To the authors' knowledge, no detailed investigation on the prevalence of H pylori in early stage gastric cancer tissue has been performed. The relationship between early stage gastric cancer and the prevalence of H pylori was studied by a immunohistochemical staining analysis.

Methods: Sixty-eight patients who were endoscopically and surgically diagnosed as having early stage gastric cancer were enrolled in this study. All tissue specimens were obtained from patients by endoscopic biopsy, and were classified histopathologically as the intestinal-type of early stage gastric cancer in 34 patients (male-to-female ratio, 28:6; age, 64 +/- 11 years) and the diffuse-type of early stage gastric cancer (male-to-female ratio, 23:11; age, 57 +/- 14 years) in the other 34 patients. The amount of H pylori in tissue samples was graded from 0 (no characteristic bacteria) to 3 (numerous bacteria) using the fluorescent microscopic and an immunohistochemical technique.

Results: Twenty-nine of the 34 cases of the intestinal-type of gastric cancer had H pylori infection, as compared with 11 of the 34 cases of diffuse-type early stage gastric cancer. A significantly higher incidence (85%; P < 0.001) of H pylori infection and, thus, higher grading scores of the number of H pylori were found in the intestinal-type early stage gastric cancer.

Conclusions: These findings suggest that the infection of H pylori may have a crucial relationship to the early stages of carcinogenesis of intestinal-type gastric cancer.

Publication types

  • Comparative Study

MeSH terms

  • Adenocarcinoma / microbiology*
  • Adenocarcinoma / pathology
  • Chronic Disease
  • Colony Count, Microbial
  • Female
  • Gastric Mucosa / microbiology
  • Gastric Mucosa / pathology
  • Gastritis / microbiology
  • Gastritis / pathology
  • Gastritis, Atrophic / microbiology
  • Gastritis, Atrophic / pathology
  • Gastroscopy
  • Helicobacter Infections / diagnosis*
  • Helicobacter pylori / isolation & purification*
  • Humans
  • Immunohistochemistry
  • Male
  • Metaplasia
  • Middle Aged
  • Neoplasm Staging
  • Staining and Labeling
  • Stomach Neoplasms / microbiology*
  • Stomach Neoplasms / pathology