Interaction of amiloride with rat parotid muscarinic and alpha-adrenergic receptors

Gen Pharmacol. 1995 Jan;26(1):155-9. doi: 10.1016/0306-3623(94)00153-e.

Abstract

1. In rat parotid acini, amiloride inhibited the secretion of amylase and the efflux of calcium and rubidium in response to carbamylcholine and to norepinephrine. 2. Amiloride competitively inhibited the binding of [3H]N-methylscopolamine and [3H] is thus a competitive antagonist of muscarinic and norepinephrine alpha-adrenergic receptors. 3. Amiloride did not affect the response to substance P with respect to secretion or ion movements. 4. Thus the Na+/H+ antiporter is not involved in the short-term regulation of amylase secretion and calcium and potassium movements in rat parotid gland function.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenergic alpha-Antagonists / pharmacology*
  • Amiloride / pharmacology*
  • Amylases / metabolism
  • Animals
  • Calcium / metabolism
  • Male
  • Muscarinic Antagonists / pharmacology*
  • Parotid Gland / drug effects*
  • Parotid Gland / metabolism
  • Potassium / metabolism
  • Propranolol / pharmacology
  • Rats
  • Rats, Wistar
  • Substance P / pharmacology

Substances

  • Adrenergic alpha-Antagonists
  • Muscarinic Antagonists
  • Substance P
  • Amiloride
  • Propranolol
  • Amylases
  • Potassium
  • Calcium