Lack of augmenting effect of interferon-gamma on dengue virus multiplication in human peripheral blood monocytes

J Med Virol. 1995 Jan;45(1):43-9. doi: 10.1002/jmv.1890450109.

Abstract

The effect of interferon-gamma (IFN-gamma) on dengue virus multiplication in human peripheral blood monocytes was investigated. Enriched monocytes were treated with IFN-gamma and then infected with dengue virus type 2 either directly or in the presence of optimal infection-enhancing levels of antibodies. Pretreatment of monocytes from dengue-immune donors with 100 IU/ml of IFN-gamma caused 12- to 97-fold and 13- to 137-fold reduction of virus yields at 24 hr after infection in the absence and presence of an anti-flavivirus monoclonal antibody, respectively. IFN-gamma also diminished virus yields when infection of monocytes from a donor who lacked anti-dengue antibody was enhanced 40-fold. The percentage of infected monocytes in IFN-gamma-pretreated cultures was similarly reduced. Dominance of the antiviral effect of IFN-gamma in monocytes is in contrast to an augmenting effect previously observed in the promonocytic cell line U937.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Antibodies, Monoclonal
  • Antigens, CD / analysis
  • Antigens, Differentiation, Myelomonocytic / analysis
  • Dengue Virus / immunology
  • Dengue Virus / physiology*
  • Flavivirus / immunology
  • Humans
  • Immune Sera / pharmacology
  • Interferon-gamma / pharmacology*
  • Lipopolysaccharide Receptors
  • Monocytes / drug effects
  • Monocytes / virology*
  • Receptors, IgG / analysis
  • Recombinant Proteins
  • Virus Replication / drug effects*

Substances

  • Antibodies, Monoclonal
  • Antigens, CD
  • Antigens, Differentiation, Myelomonocytic
  • Immune Sera
  • Lipopolysaccharide Receptors
  • Receptors, IgG
  • Recombinant Proteins
  • Interferon-gamma