All you wanted to know about SELEX

Mol Biol Rep. 1994;20(2):97-107. doi: 10.1007/BF00996358.


In vitro selection, or SELEX, is a technique that allows the simultaneous screening of highly diverse pools of different RNA or DNA (dsDNA or ssDNA) molecules for a particular feature. Different examples from a great variety of applications of in vitro selection experiments are described and a detailed overview of the method and its variations will be given. Some especially conclusive in vitro selection experiments are discussed in detail to illustrate the potential power and diversity of this method. Potential restrictions of the methods and possible ways to overcome them are pointed out.

Publication types

  • Review

MeSH terms

  • Base Sequence
  • Biological Evolution
  • Chromatography, Affinity
  • DNA / chemistry*
  • DNA / genetics
  • DNA / isolation & purification
  • DNA Primers
  • DNA-Binding Proteins
  • Ligands
  • Molecular Biology / methods*
  • Molecular Sequence Data
  • Molecular Structure
  • RNA / chemistry*
  • RNA / genetics
  • RNA / isolation & purification
  • RNA, Catalytic / isolation & purification
  • RNA-Binding Proteins


  • DNA Primers
  • DNA-Binding Proteins
  • Ligands
  • RNA, Catalytic
  • RNA-Binding Proteins
  • RNA
  • DNA