Specific DNA-RNA hybrid binding by zinc finger proteins

Science. 1995 Apr 14;268(5208):282-4. doi: 10.1126/science.7536342.


Zinc finger proteins of the Cys2His2 type represent a large class of proteins that have been assumed to function by means of specific interactions with DNA. Experiments motivated by structural characteristics of zinc finger protein-DNA complexes revealed that certain zinc finger proteins bound DNA-RNA hybrids with affinities comparable to or greater than those for DNA duplexes. The interactions between the zinc finger proteins and the DNA-RNA hybrids were dependent on which strand was RNA and were sequence-specific. Thus, interactions with DNA-RNA hybrids should be considered with regard to the biological roles of zinc finger proteins.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Binding Sites
  • DNA / chemistry
  • DNA / metabolism*
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • RNA / chemistry
  • RNA / metabolism*
  • RNA-Binding Proteins / chemistry
  • RNA-Binding Proteins / metabolism*
  • Sp1 Transcription Factor / metabolism
  • Zinc Fingers / physiology*


  • RNA-Binding Proteins
  • Sp1 Transcription Factor
  • RNA
  • DNA