Fas antigen and bcl-2 expression on lymphocytes cultured with cytomegalovirus and varicella-zoster virus antigen

Cell Immunol. 1995 Feb;160(2):173-7. doi: 10.1016/0008-8749(95)80024-d.

Abstract

Fas antigen and bcl-2 expression on peripheral blood mononuclear cells (PBMC) cultured with cytomegalovirus (CMV) and varicella-zoster virus (VZV) antigen was analyzed by three-color flow cytometry. Expression of Fas antigen increased significantly in CD45RO+ populations of both CD4+ and CD8+ cells obtained from CMV and VZV seropositive donors after culture with CMV and VZV antigen for 6 days. Fas antigen expression did not increase in PBMC cultured with control antigen. In contrast, bcl-2 expression decreased both in CD4+CD45RO+ and CD8+CD45RO+ cells from the same donors. Fas antigen and bcl-2 expression in CD45RA+ populations did not change. Cell viability of cultured cells with CMV and VZV antigen decreased after treatment with anti-Fas antibody and DNA fragments indicating apoptosis were detected in the cell lysate of these cultured cells after treatment with anti-Fas antibody. These data suggest that Fas antigen and bcl-2 protein may interact in regulating the cell death process of activated memory lymphocytes and eliminating lymphocytes activated by viral infection.

MeSH terms

  • Adult
  • Antibodies, Monoclonal / immunology
  • Antibodies, Monoclonal / pharmacology
  • Antibodies, Viral / blood
  • Antigens, Surface / immunology
  • Antigens, Surface / metabolism*
  • Antigens, Viral / pharmacology*
  • Apoptosis / physiology*
  • Cells, Cultured
  • Cytomegalovirus / immunology
  • Cytomegalovirus / physiology*
  • DNA / analysis
  • Gene Expression Regulation* / drug effects
  • Herpesvirus 3, Human / immunology*
  • Herpesvirus 3, Human / physiology
  • Humans
  • Lymphocyte Activation
  • Lymphocyte Subsets / metabolism*
  • Lymphocyte Subsets / virology
  • Proto-Oncogene Proteins / metabolism*
  • Proto-Oncogene Proteins c-bcl-2
  • fas Receptor

Substances

  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Antigens, Surface
  • Antigens, Viral
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • fas Receptor
  • DNA