Identification of beta-1,6-glucosylated cell wall proteins in yeast and hyphal forms of Candida albicans

Eur J Cell Biol. 1994 Dec;65(2):402-7.


Several cell wall proteins released from yeast and hyphal cells of Candida albicans by laminarinase reacted with an affinity-purified antiserum raised against beta-1,6-glucan. Binding of the antiserum was competitively inhibited by beta-1,6-glucan, but not by beta-1,3-glucan or isolated N-chains. Immunodetection was completely abolished when the proteins were treated with periodate. These results demonstrate that the laminarinase-released wall proteins of C. albicans possess an epitope consisting of beta-1,6-linked glucose residues. The yeast form of C. albicans contained four beta-1,6-glucosylated wall proteins, an Endo H-resistant protein of 125 kDa and three glycoproteins which became only detectable after Endo H digestion and had a molecular mass of 320, 170 and 44 kDa, respectively. As for the hyphal form, a different set of beta-1,6-glucosylated wall proteins was found consisting of two Endo H-resistant glycoproteins of 125 and 80 kDa, respectively, and two glycoproteins that after Endo H digestion had a molecular mass of 320 and 38 kDa, respectively. Sodium dodecyl sulfate-extractable wall proteins and medium proteins did not react with the beta-1,6-glucan antiserum. The beta-1,6-glucan epitope could be removed by aqueous hydrofluoric acid indicating that the epitope is phosphodiester-linked to the cell wall proteins. It is speculated that the epitope forms part of a GPI-anchor and might be involved in the anchoring of mannoproteins into the cell wall.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Candida albicans / chemistry*
  • Candida albicans / ultrastructure
  • Cell Wall / chemistry
  • Epitopes / analysis
  • Fungal Proteins / analysis*
  • Glucans / metabolism*
  • Glycosylation
  • Membrane Glycoproteins / analysis*
  • beta-Glucans*


  • Epitopes
  • Fungal Proteins
  • Glucans
  • Membrane Glycoproteins
  • beta-Glucans
  • mannoproteins
  • beta-1,6-glucan