Chemokine expression in murine experimental allergic encephalomyelitis

J Neuroimmunol. 1995 May;58(2):167-76. doi: 10.1016/0165-5728(95)00008-p.


Chemokines are a family of low molecular mass proteins with chemotactic and cell activating activities. Reverse transcription-polymerase chain reaction and Northern hybridization were used to examine their expression during murine experimental allergic encephalomyelitis (EAE), an autoimmune disease used as a model of multiple sclerosis. The mRNAs encoding RANTES, MIP-1 alpha, MIP-1 beta, TCA3 (I-309), IP-10, JE (MCP-1), KC (MGSA/gro), and MARC (MCP-3) were induced in the spinal cord 1-2 days before clinical signs were apparent. SDF, a cDNA predicted to encode a chemokine-like product, was expressed in normal as well as diseased spinal cords. No expression of C10 or MIP-2 was detected. Activated encephalitogenic T cells expressed message for RANTES, MIP-1 alpha, MIP-1 beta, and TCA3. These results define a subset of chemokines that may play an important role in the inflammatory process during murine EAE.

MeSH terms

  • Animals
  • Base Sequence
  • Chemokine CCL1
  • Chemokine CCL4
  • Chemokine CCL5
  • Chemokine CCL7
  • Chemokines, CC
  • Chemotactic Factors / genetics*
  • Chemotactic Factors / metabolism
  • Cytokines / genetics
  • Cytokines / metabolism
  • Encephalomyelitis, Autoimmune, Experimental / genetics*
  • Encephalomyelitis, Autoimmune, Experimental / immunology
  • Encephalomyelitis, Autoimmune, Experimental / metabolism
  • Female
  • Lymphokines / genetics
  • Macrophage Inflammatory Proteins
  • Mice
  • Molecular Sequence Data
  • Monocyte Chemoattractant Proteins*
  • Monokines / genetics
  • Monokines / metabolism
  • Myelin Proteins / immunology
  • Myelin Proteolipid Protein*
  • Peptide Fragments / immunology
  • Polymerase Chain Reaction
  • RNA-Directed DNA Polymerase
  • Receptors, CCR8


  • CCL1 protein, human
  • CCL7 protein, human
  • Ccl7 protein, mouse
  • Ccr8 protein, mouse
  • Chemokine CCL1
  • Chemokine CCL4
  • Chemokine CCL5
  • Chemokine CCL7
  • Chemokines, CC
  • Chemotactic Factors
  • Cytokines
  • Lymphokines
  • Macrophage Inflammatory Proteins
  • Monocyte Chemoattractant Proteins
  • Monokines
  • Myelin Proteins
  • Myelin Proteolipid Protein
  • Peptide Fragments
  • Receptors, CCR8
  • myelin proteolipid protein (139-151)
  • RNA-Directed DNA Polymerase