Transforming growth factor beta 1 inhibits IL-3- and IL-4-dependent mouse connective tissue-type mast cell proliferation

Arch Dermatol Res. 1995;287(2):198-201. doi: 10.1007/BF01262332.


Transforming growth factor beta 1 (TGF beta 1) is a regulator of cell proliferation and differentiation. Using a mouse peritoneal cell-derived mast cell culture system, we investigated the effects of TGF beta 1 on mast cell proliferation. TGF beta 1 inhibited IL-3- and IL-4-dependent connective tissue-type mast cell proliferation. The effect was concentration dependent: 50% inhibition was observed with 1.0 ng/ml TGF beta 1 and the maximal inhibitory effect (no proliferation), was observed with 10 ng/ml. Flow cytometric analysis suggested that the inhibitory effect of TGF beta 1 was due to blocking of both G1 and G2 phases. Both control and TGF beta 1-treated mast cells showed similar histamine release induced by the calcium ionophore, A23187. TGF beta 1 seems to be an important negative regulator of connective tissue-type mast cell proliferation with apparently no appreciable effect on mast cell function.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Calcimycin / pharmacology
  • Cell Cycle / drug effects*
  • Cell Division / drug effects
  • Cells, Cultured
  • Connective Tissue Cells*
  • Dose-Response Relationship, Drug
  • Flow Cytometry
  • Histamine Release / drug effects
  • Humans
  • Interleukin-3 / physiology*
  • Interleukin-4 / physiology*
  • Mast Cells / cytology*
  • Mast Cells / drug effects
  • Mice
  • Mice, Inbred Strains
  • Recombinant Proteins / pharmacology
  • Transforming Growth Factor beta / pharmacology*


  • Interleukin-3
  • Recombinant Proteins
  • Transforming Growth Factor beta
  • Interleukin-4
  • Calcimycin