Pyrrolidine dithiocarbamate prevents IL-1-induced nitric oxide synthase mRNA, but not superoxide dismutase mRNA, in insulin producing cells

Biochem Biophys Res Commun. 1995 May 25;210(3):816-22. doi: 10.1006/bbrc.1995.1731.

Abstract

We presently investigated the effects of pyrrolidine dithiocarbamate (PDTC), a potent inhibitor of nuclear factor kappa B (NF-kappa B), on the induction of nitric oxide synthase (iNOS) and manganese superoxide dismutase (MnSOD) mRNAs by IL-1 beta in insulin-producing RIN cells. PDTC decreased by 90% both IL-1 beta-induced increase in medium nitrite accumulation (an indicator of NO production) and induction of iNOS mRNA expression. However, PDTC did not prevent induction of MnSOD mRNA by IL-1 beta. PDTC induced an early (45 min) expression of c-fos mRNA and potentiated IL-1 beta-induced c-fos expression. Our data suggest that NF-kappa B activation is necessary for iNOS, but not for MnSOD, mRNA expression in insulin producing cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Oxidoreductases / biosynthesis*
  • Animals
  • Antioxidants / pharmacology*
  • Enzyme Induction / drug effects
  • Gene Expression / drug effects*
  • Genes, fos
  • Humans
  • Insulin / biosynthesis
  • Insulinoma
  • Interleukin-1 / antagonists & inhibitors
  • Interleukin-1 / pharmacology*
  • Kinetics
  • Nitric Oxide Synthase
  • Pancreatic Neoplasms
  • Proto-Oncogene Proteins c-fos / biosynthesis
  • Pyrrolidines / pharmacology*
  • RNA, Messenger / biosynthesis
  • Recombinant Proteins / pharmacology
  • Superoxide Dismutase / biosynthesis*
  • Thiocarbamates / pharmacology*
  • Tumor Cells, Cultured

Substances

  • Antioxidants
  • Insulin
  • Interleukin-1
  • Proto-Oncogene Proteins c-fos
  • Pyrrolidines
  • RNA, Messenger
  • Recombinant Proteins
  • Thiocarbamates
  • pyrrolidine dithiocarbamic acid
  • Nitric Oxide Synthase
  • Superoxide Dismutase
  • Amino Acid Oxidoreductases