The role of gamma delta T cells in priming macrophages to produce tumor necrosis factor-alpha

Eur J Immunol. 1995 May;25(5):1465-8. doi: 10.1002/eji.1830250551.


The secretion of tumor necrosis factor (TNF)-alpha from macrophages is regulated by both priming and triggering signals. We found that macrophages from mice lacking gamma delta T cells [T cell receptor (TCR) delta-/- mice], which lack the gene encoding the delta chain, produced only small amounts of TNF-alpha in response to lipopolysaccharide (LPS) and showed a reduced level of expression of CD14. Pre-incubation of macrophages from TCR delta-/- mice with gamma delta T cells from their TCR delta +/- littermates restored their capacity to produce TNF-alpha in response to LPS. The priming activity of gamma delta T cells was in part inhibited by neutralizing anti-interferon (IFN)-gamma monoclonal antibodies. Collectively, these results suggest that gamma delta T cells play a role in priming macrophages to a steady state of activation via IFN-gamma secretion, which allows them to produce TNF-alpha when exposed to LPS.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antibodies, Monoclonal / pharmacology
  • Antigens, CD / biosynthesis
  • Antigens, CD / genetics
  • Antigens, Differentiation, Myelomonocytic / biosynthesis
  • Antigens, Differentiation, Myelomonocytic / genetics
  • Chimera
  • Interferon-gamma / antagonists & inhibitors
  • Interferon-gamma / immunology
  • Interferon-gamma / metabolism
  • Lipopolysaccharide Receptors
  • Lipopolysaccharides / pharmacology
  • Lymphocyte Activation
  • Macrophage Activation*
  • Macrophages, Peritoneal / drug effects
  • Macrophages, Peritoneal / metabolism
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Knockout
  • Receptors, Antigen, T-Cell, gamma-delta / analysis*
  • Receptors, Antigen, T-Cell, gamma-delta / deficiency
  • Receptors, Antigen, T-Cell, gamma-delta / genetics
  • Signal Transduction*
  • Specific Pathogen-Free Organisms
  • T-Lymphocyte Subsets / immunology*
  • Tumor Necrosis Factor-alpha / biosynthesis*
  • Tumor Necrosis Factor-alpha / genetics
  • Tumor Necrosis Factor-alpha / metabolism


  • Antibodies, Monoclonal
  • Antigens, CD
  • Antigens, Differentiation, Myelomonocytic
  • Lipopolysaccharide Receptors
  • Lipopolysaccharides
  • Receptors, Antigen, T-Cell, gamma-delta
  • Tumor Necrosis Factor-alpha
  • Interferon-gamma