Increased expression of adhesion molecules (CD54, CD29 and CD44) on fibroblasts infected with cytomegalovirus

Microbiol Immunol. 1995;39(2):129-33. doi: 10.1111/j.1348-0421.1995.tb02179.x.


The expression of ICAM-1 (CD54), beta 1 integrin (CD29), and CD44 on cytomegalovirus (CMV)-infected human embryonic fibroblasts (HEF) was analyzed by flow cytometry. The expression of these adhesion molecules increased significantly on CMV-infected HEF, on days 2 and 5 after inoculation, compared to uninfected HEF. However, the expression of these adhesion molecules decreased on herpes simplex virus (HSV)-1 and varicella-zoster virus (VZV)-infected HEF. Increased expression was not observed on HEF treated either with inactivated CMV or with supernatant fluid of CMV-infected cells. The addition of anti-cytokine (TNF-alpha, IL-1 beta, or IFN-gamma) antibodies had no effect on the increase of these adhesion molecules. This suggests that the increase in CD54, CD29, and CD44 on CMV-infected cells requires active virus replication and was not mediated by a soluble factor released from CMV-infected cells. Changes in adhesion molecules on CMV-infected fibroblasts may contribute to inflammation induced by CMV infection.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / pharmacology
  • Antigens, CD / biosynthesis*
  • Carrier Proteins / biosynthesis*
  • Cells, Cultured
  • Chlorocebus aethiops
  • Culture Media
  • Cytokines / immunology
  • Cytomegalovirus / growth & development*
  • Fibroblasts / metabolism*
  • Fibroblasts / microbiology
  • Flow Cytometry
  • Herpesvirus 1, Human / growth & development
  • Herpesvirus 3, Human / growth & development
  • Humans
  • Hyaluronan Receptors
  • Integrin beta1
  • Integrins / biosynthesis*
  • Intercellular Adhesion Molecule-1 / biosynthesis*
  • Receptors, Cell Surface / biosynthesis*
  • Receptors, Lymphocyte Homing / biosynthesis*
  • Vero Cells
  • Virus Replication


  • Antibodies, Monoclonal
  • Antigens, CD
  • Carrier Proteins
  • Culture Media
  • Cytokines
  • Hyaluronan Receptors
  • Integrin beta1
  • Integrins
  • Receptors, Cell Surface
  • Receptors, Lymphocyte Homing
  • Intercellular Adhesion Molecule-1