CSF-1 treatment promotes angiogenesis in the metaphysis of osteopetrotic (toothless, tl) rats

Bone. 1995 Mar;16(3):315-24. doi: 10.1016/8756-3282(94)00044-1.


It has recently been shown that following treatment with colony-stimulating factor-1 (CSF-1) the osteopetrotic condition in toothless (tl) rats greatly improves and growth is accelerated. We have examined the effects of such treatment on the microvasculature of the distal femoral chondro-osseous junction, a site where bone growth in length is coordinated with angiogenesis. Vascular casts and ultrastructural analyses of this region showed that, compared to untreated normal rats, untreated mutants showed little bone growth or angiogenesis. When mutants were treated with CSF-1 angiogenesis was markedly accelerated. These data show a remarkable effect of this growth factor on angiogenesis in this osteopetrotic mutation. Whether this effect of CSF-1 on angiogenesis is direct or indirect is not known and indicates that its effects on the normal microvasculature deserve further study.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Arterioles / drug effects
  • Arterioles / growth & development
  • Arterioles / ultrastructure
  • Capillaries / drug effects
  • Capillaries / growth & development
  • Capillaries / ultrastructure
  • Cartilage / cytology
  • Cartilage / drug effects
  • Cartilage / ultrastructure
  • Corrosion Casting
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / drug effects
  • Endothelium, Vascular / ultrastructure
  • Female
  • Femur / blood supply*
  • Femur / drug effects
  • Femur / ultrastructure
  • Macrophage Colony-Stimulating Factor / administration & dosage
  • Macrophage Colony-Stimulating Factor / adverse effects*
  • Macrophage Colony-Stimulating Factor / therapeutic use
  • Male
  • Microscopy, Electron
  • Microscopy, Electron, Scanning
  • Mutation / genetics
  • Neovascularization, Pathologic / chemically induced*
  • Osteopetrosis / drug therapy*
  • Osteopetrosis / genetics
  • Rats
  • Rats, Mutant Strains


  • Macrophage Colony-Stimulating Factor