Prevalence and activation phenotype of Sendai virus-specific CD4+ T cells

Virology. 1995 Jun 20;210(1):179-85. doi: 10.1006/viro.1995.1329.


The relative prevalence of Sendai virus-specific CD4+ T helper precursors (Thp) has been assessed by limiting dilution analysis (LDA). Within 10 days after intranasal inoculation of C57BL/6 mice, Thp prevalence increased from < 1 in 10(5) to approximately 1 in 200 in CD4+ T-cell-enriched spleen and mediastinal lymph node populations. These frequencies remained elevated relative to naive animals, with the majority of the Thp recovered at 2-3 months after infection being found in the spleen. The "memory" Thp express an "activated" L-selectin-low, CD44-high, alpha 4-integrin-high phenotype, comparable to that described previously for CD8+ cytotoxic T lymphocyte precursors (CTLp) specific for Sendai virus. Background effects for the IL-2-based Thp analysis are, however, less predictable than those found previously for the 51Cr release CTLp LDA, as the extent of non-virus-specific lymphokine production varies. However, provided the analysis is appropriately controlled, the approach does allow useful comparisons between phenotypically different subsets of antigen-specific CD4+ T cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigen-Presenting Cells / immunology
  • CD4 Antigens / analysis
  • CD4-Positive T-Lymphocytes / immunology*
  • CD4-Positive T-Lymphocytes / virology*
  • Carrier Proteins / analysis
  • Cells, Cultured
  • Female
  • Hyaluronan Receptors
  • Immunophenotyping
  • Influenza A virus / immunology*
  • Leukocyte Common Antigens / analysis
  • Lymphocyte Activation*
  • Lymphocyte Depletion
  • Mice
  • Mice, Inbred C57BL
  • Orthomyxoviridae Infections / immunology*
  • Parainfluenza Virus 1, Human / immunology*
  • Receptors, Cell Surface / analysis
  • Receptors, Lymphocyte Homing / analysis
  • Regression Analysis
  • Spleen / immunology
  • Time Factors


  • CD4 Antigens
  • Carrier Proteins
  • Hyaluronan Receptors
  • Receptors, Cell Surface
  • Receptors, Lymphocyte Homing
  • Leukocyte Common Antigens