High D-glucose stimulates the cell cycle from the G1 to the S and M phases, but has no competent effect on the G0 phase, in vascular smooth muscle cells

Biochem Biophys Res Commun. 1995 Jun 15;211(2):619-26. doi: 10.1006/bbrc.1995.1858.


The effects of high (28mM) D-glucose (HG) on the cell cycle progression were investigated in rat aorta vascular smooth muscle cells (VSMCs) in primary culture, using an immunocytochemical analysis of cell-cycle-specific nuclear antigens. HG had no effect on the cell cycle of the serum-deprived G0 cells, whereas platelet-derived growth factor (PDGF) stimulated the entry of the G0 cells to the G1 phase without a further progression to the S and M phases. HG, but neither mannitol nor L-glucose, stimulated the progression of the PDGF-pretreated G1 cells to the S and M phases, which was blocked by calphostin-C, a protein kinase C (PKC) blocker. HG did not affect the cytosolic Ca2+ concentration ([Ca2+]i). These data suggest that HG has no competent effect on the G0 cells and acts as a progression growth factor (to stimulate the cell cycle from the G1 to the S/M) in VSMCs through the mechanism, which may be insensitive to [Ca2+]i and mediated by PKC.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aorta / cytology
  • Aorta / drug effects
  • Becaplermin
  • Cell Cycle / drug effects*
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Fluorescent Antibody Technique
  • G1 Phase
  • Glucose / pharmacology*
  • Humans
  • Ki-67 Antigen
  • Kinetics
  • Male
  • Mannitol / pharmacology
  • Mitosis
  • Muscle, Smooth, Vascular / cytology*
  • Muscle, Smooth, Vascular / drug effects
  • Naphthalenes*
  • Neoplasm Proteins / analysis
  • Nickel / pharmacology
  • Nuclear Proteins / analysis
  • Platelet-Derived Growth Factor / pharmacology
  • Polycyclic Compounds / pharmacology
  • Proliferating Cell Nuclear Antigen / analysis
  • Protein Kinase C / antagonists & inhibitors
  • Proto-Oncogene Proteins c-sis
  • Rats
  • Rats, Wistar
  • Recombinant Proteins / pharmacology
  • Resting Phase, Cell Cycle
  • S Phase
  • Stereoisomerism
  • Time Factors


  • Ki-67 Antigen
  • Naphthalenes
  • Neoplasm Proteins
  • Nuclear Proteins
  • Platelet-Derived Growth Factor
  • Polycyclic Compounds
  • Proliferating Cell Nuclear Antigen
  • Proto-Oncogene Proteins c-sis
  • Recombinant Proteins
  • Becaplermin
  • Mannitol
  • nickel chloride
  • Nickel
  • Protein Kinase C
  • calphostin C
  • Glucose