Upregulation of interleukin-8 receptor in human polymorphonuclear neutrophils by formyl peptide and lipopolysaccharide

FEBS Lett. 1995 Jun 26;367(2):117-21. doi: 10.1016/0014-5793(95)00525-e.


Interleukin-8 (IL-8) is implicated in the pathogenesis of a large number of neutrophil-driven inflammatory diseases. Although the cytokine activates neutrophils through a receptor, no information is available regarding the regulation of IL-8 receptor (IL-8R) expression. The present study shows that, compared to control, the bacterial products--formylpeptide and LPS (serum-activated) upregulate IL-8 receptor by 54% and 115%, respectively, the former by degranulation of the secretory vesicle and the latter by de novo protein synthesis. The newly expressed IL-8R could be demonstrated with anti-IL-8R-antibody and by autoradiogram of the receptor crosslinked with [125I]IL-8. The study may be useful for understanding the potential role of IL-8 during neutrophil mediated inflammatory response.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies / immunology
  • Antigens, CD / immunology
  • Antigens, Differentiation, Myelomonocytic / immunology
  • Cross-Linking Reagents
  • Humans
  • Interleukin-8 / metabolism
  • Lipopolysaccharide Receptors
  • Lipopolysaccharides / pharmacology*
  • N-Formylmethionine Leucyl-Phenylalanine / pharmacology*
  • Neutrophil Activation
  • Neutrophils / drug effects
  • Neutrophils / metabolism*
  • Polymyxin B / pharmacology
  • Receptors, Interleukin / immunology
  • Receptors, Interleukin / metabolism*
  • Receptors, Interleukin-8A
  • Recombinant Proteins / metabolism
  • Up-Regulation


  • Antibodies
  • Antigens, CD
  • Antigens, Differentiation, Myelomonocytic
  • Cross-Linking Reagents
  • Interleukin-8
  • Lipopolysaccharide Receptors
  • Lipopolysaccharides
  • Receptors, Interleukin
  • Receptors, Interleukin-8A
  • Recombinant Proteins
  • N-Formylmethionine Leucyl-Phenylalanine
  • Polymyxin B