Characterization of cord-blood-derived human mast cells cultured in the presence of Steel factor and interleukin-6

Int Arch Allergy Immunol. May-Jun 1995;107(1-3):63-5. doi: 10.1159/000236932.

Abstract

We generated > 10(7) mast cells by culturing 10(7) cord blood mononuclear cells for > 10 weeks in the presence of Steel factor, interleukin-6 and prostaglandin E2. 99% of the cultured cells had tryptase-positive granules, while 18% had chymase-positive granules. Cultured mast cells contained 3.6 micrograms histamine and 3.5 micrograms tryptase per 10(6) cells. Cells sensitized with 1 microgram/ml human IgE released 58.5% histamine and 1.55 ng tumor necrosis factor (TNF)-alpha per 10(6) cells when challenged with 1 microgram/ml antihuman IgE, whereas the control cells spontaneously released 3.7% histamine and 0.18 ng TNF-alpha. Analysis for surface antigens revealed that cultured mast cells expressed the following CD molecules: 9, 13, 14, 29, 33, 38, 43, 44, 45RA, 45RB, 46, 47, 48, 49d, 50, 51, 53, 54, 55, 58, 59, 60, 61 and 117 (c-Kit). Taken together, these cultured cells seem to be functionally mature mast cells.

MeSH terms

  • Antigens, CD / analysis
  • Cell Differentiation / drug effects
  • Cells, Cultured
  • Chymases
  • Cytoplasmic Granules / enzymology
  • Dinoprostone / pharmacology
  • Fetal Blood / cytology*
  • Hematopoietic Cell Growth Factors / pharmacology*
  • Histamine / pharmacology
  • Humans
  • Immunoglobulin E / pharmacology
  • Interleukin-6 / pharmacology*
  • Mast Cells / cytology
  • Mast Cells / drug effects*
  • Mast Cells / enzymology
  • Recombinant Proteins / pharmacology
  • Serine Endopeptidases / analysis
  • Stem Cell Factor
  • Tryptases
  • Tumor Necrosis Factor-alpha / pharmacology

Substances

  • Antigens, CD
  • Hematopoietic Cell Growth Factors
  • Interleukin-6
  • Recombinant Proteins
  • Stem Cell Factor
  • Tumor Necrosis Factor-alpha
  • Immunoglobulin E
  • Histamine
  • Serine Endopeptidases
  • chymase 2
  • Chymases
  • Tryptases
  • Dinoprostone