Failure of B-cell differentiation in mice lacking the transcription factor EBF

Nature. 1995 Jul 20;376(6537):263-7. doi: 10.1038/376263a0.


Early B-cell factor (EBF) is a cell type-specific transcription factor that is expressed at all antigen-independent stages of B-lymphocyte differentiation and participates in the regulation of the mb-1 gene. Here we show, by targeted gene disruption in mice, that EBF is necessary for the generation of immunoglobulin-expressing B cells. EBF-deficient mice lack B cells that have rearranged their immunoglobulin D and JH gene segments, but contain B220+CD43+ progenitor cells that express germline mu and IL-7 receptor transcripts. Various non-lymphoid tissues that express EBF are apparently normal in homozygous mutant mice, including olfactory neurons in which EBF was identified as Olf-1 (refs 5, 6). Together, these data suggest that EBF plays a specific and important role in the transcriptional control of B-cell differentiation at a stage before Ig (immunoglobulin) gene rearrangement but after commitment of cells to the B-lymphoid lineage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD / genetics
  • Antigens, Surface / immunology
  • B-Lymphocytes / cytology*
  • B-Lymphocytes / immunology
  • Base Sequence
  • CD79 Antigens
  • Cell Differentiation / physiology
  • DNA Primers
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / physiology*
  • Flow Cytometry
  • Gene Rearrangement, B-Lymphocyte
  • Leukocyte Common Antigens
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Mutagenesis
  • Receptors, Antigen, B-Cell / genetics
  • Trans-Activators / deficiency
  • Trans-Activators / genetics
  • Trans-Activators / physiology*


  • Antigens, CD
  • Antigens, Surface
  • CD79 Antigens
  • Cd79a protein, mouse
  • DNA Primers
  • DNA-Binding Proteins
  • Ebf1 protein, mouse
  • Receptors, Antigen, B-Cell
  • Trans-Activators
  • Leukocyte Common Antigens