Distinct roles of L-selectin and integrins alpha 4 beta 7 and LFA-1 in lymphocyte homing to Peyer's patch-HEV in situ: the multistep model confirmed and refined

Immunity. 1995 Jul;3(1):99-108. doi: 10.1016/1074-7613(95)90162-0.


Circulating lymphocytes home to the mucosal lymphoid organs, Peyer's patches (PP), through high endothelial venules (HEV). In situ analyses revealed that transfused lymph node cells (LNCs) interact with PP-HEV in a series of overlapping adhesion events: L-selectin (CD62L) > alpha 4 beta 7 initiates interaction, L-selectin and alpha 4 beta 7 both participate in rolling, and G alpha i-linked activation triggers arrest that requires both alpha 4 beta 7 and LFA-1. alpha 4 beta 7 dramatically reduces rolling velocity, and appears to be required for engagement of LFA-1. In contrast with resting LNC, preactivated LNC or alpha 4 beta 7hi lymphoma cells require only alpha 4 beta 7 for arrest in PP-HEV. The predominant PP-HEV ligand for alpha 4 beta 7 but also apparently for L-selectin is the mucosal addressin MAd-CAM-1. These results validate the concept of multimolecular adhesion/decision cascades in physiologic lymphocyte-endothelial recognition, define a novel role for alpha 4 integrins as a "bridge" between selectin and beta 2 integrin-dependent events, and reemphasize the potential for direct adhesion through preactivated alpha 4 integrins alone.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, CD / immunology*
  • Cell Adhesion
  • Cell Adhesion Molecules / immunology*
  • Cell Movement
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / immunology
  • Integrin alpha4
  • L-Selectin
  • Lymphocyte Function-Associated Antigen-1 / immunology*
  • Lymphocytes / immunology*
  • Mice
  • Microscopy, Video
  • Peyer's Patches / blood supply
  • Peyer's Patches / cytology
  • Peyer's Patches / immunology*
  • Receptors, Lymphocyte Homing / immunology
  • Veins / cytology


  • Antigens, CD
  • Cell Adhesion Molecules
  • Lymphocyte Function-Associated Antigen-1
  • Receptors, Lymphocyte Homing
  • L-Selectin
  • Integrin alpha4