B7.2 provides co-stimulatory functions in vivo in response to staphylococcal enterotoxin B

Eur J Immunol. 1995 Jul;25(7):2111-4. doi: 10.1002/eji.1830250747.

Abstract

Excessive T cell activation induced by bacterial superantigens plays an important role in the pathology associated with Gram-positive bacteremia. To gain insight into the early phases of T cell activation by bacterial enterotoxins in vivo, we investigated the ability of antibodies to well-defined co-stimulatory molecules to inhibit T cell activation and the subsequent toxic shock syndrome induced in BALB/c mice following the injection of staphylococcal enterotoxin B (SEB). We demonstrate here that a single dose of anti-B7.2 antibodies, but not anti-B7.1 antibodies, significantly inhibits T cell activation, as judged by lower systemic IL-2 release, blastogenesis and IL-2 receptor expression, and reduces the lethal effect of SEB in D-galactosamine-sensitized mice. These results demonstrate that co-stimulation through the B7.2 molecule plays an important role in the activation of T cells in response to SEB in vivo and suggest alternative therapies for septic shock caused by bacterial enterotoxins based on blocking antibodies to co-stimulatory molecules.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antigens, CD*
  • B7-1 Antigen / physiology*
  • B7-2 Antigen
  • Clonal Anergy
  • Enterotoxins / antagonists & inhibitors
  • Enterotoxins / toxicity*
  • Female
  • Lymphocyte Activation
  • Membrane Glycoproteins / physiology*
  • Mice
  • Mice, Inbred BALB C
  • Spleen / cytology
  • T-Lymphocytes / immunology*

Substances

  • Antibodies, Monoclonal
  • Antigens, CD
  • B7-1 Antigen
  • B7-2 Antigen
  • Cd86 protein, mouse
  • Enterotoxins
  • Membrane Glycoproteins
  • enterotoxin B, staphylococcal