Detection of multiple gains and losses of genetic material in ten glioma cell lines by comparative genomic hybridization

Genes Chromosomes Cancer. 1995 Jun;13(2):86-93. doi: 10.1002/gcc.2870130203.

Abstract

A protocol for comparative genomic hybridization by use of nucleotides directly labeled with fluorochromes was used to map regions of deletion and amplification in ten glioma cell lines. The protocol greatly reduced experimental artifacts. We detected several genetic aberrations, including whole chromosome loss and gain, partial loss and gain, possible isochromosome, and higher level DNA amplification. The most frequent losses (in order of frequency) occurred on chromosomes 10, 18, 13, 11, 9, 14, 4, 6, 1, and X. The most common gain occurred on chromosome 7. Several sites of previously known and unknown amplifications were observed.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Brain Neoplasms / genetics*
  • Chromosome Aberrations / genetics*
  • Chromosome Mapping
  • DNA, Neoplasm / analysis
  • Female
  • Glioma / genetics*
  • Humans
  • Male
  • Metaphase
  • Nucleic Acid Hybridization
  • Tumor Cells, Cultured

Substances

  • DNA, Neoplasm