Impairment of T-cell-dependent B-cell responses and B-1 cell development in CD19-deficient mice

Nature. 1995 Jul 27;376(6538):352-5. doi: 10.1038/376352a0.


CD19 is the hallmark differentiation antigen of the B lineage. Its early expression has implicated a role for CD19 during the antigen-independent phases of B-cell development, whereas in mature B cells CD19 can act synergistically with surface immunoglobulin to induce activation. We have generated CD19-deficient mice and found that development of conventional B cells is unperturbed. However, mature CD19-/- B cells show a profound deficiency in responding to protein antigens that require T-cell help. This is accompanied by a lack of germinal centre formation and affinity maturation of serum antibodies. Thus CD19 is crucial for both initial B-cell activation by T-cell-dependent antigens and the maturation and/or selection of the activated cells into the memory compartment. An impairment in ligand-driven selection may also be responsible for the observation of a striking reduction in the B-1 (formerly Ly-1) B-cell subset, thought to develop under the control of self-antigens and bacterial antigens (reviewed in ref. 2).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens / immunology
  • Antigens, CD / immunology*
  • Antigens, CD19
  • Antigens, Differentiation, B-Lymphocyte / immunology*
  • B-Lymphocyte Subsets / cytology
  • B-Lymphocyte Subsets / immunology*
  • B-Lymphocytes / cytology
  • B-Lymphocytes / immunology*
  • Base Sequence
  • Bone Marrow Cells
  • Cell Differentiation
  • Cells, Cultured
  • DNA Primers
  • Flow Cytometry
  • Hematopoietic Stem Cells
  • Lymphocyte Activation / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Mutagenesis
  • Peritoneal Cavity / cytology
  • Spleen / cytology
  • T-Lymphocytes / immunology*


  • Antigens
  • Antigens, CD
  • Antigens, CD19
  • Antigens, Differentiation, B-Lymphocyte
  • DNA Primers