A common RNA loop motif as a docking module and its function in the hammerhead ribozyme

Nat Struct Biol. 1994 Nov;1(11):820-7. doi: 10.1038/nsb1194-820.

Abstract

Here I present a three-dimensional model of a novel element of RNA tertiary structure. A common loop motif composed of adjacent, sheared G.A and A.N non-canonical base pairs is proposed to form long-range tertiary interactions with other RNA residues. The widespread distribution of this G.A/A.N docking module suggests that the putative long-range docking interaction plays an important role in specifying the tertiary structure of large RNAs, and perhaps the quaternary structure of some intermolecular RNA-RNA interactions. Application of this docking module hypothesis to the hammerhead ribozyme provides crucial constraints for the calculation of three-dimensional models of its self-cleaving conformation.

MeSH terms

  • Base Sequence
  • Models, Molecular
  • Molecular Sequence Data
  • Nucleic Acid Conformation*
  • RNA / chemistry*
  • RNA / metabolism
  • RNA, Catalytic / chemistry*
  • RNA, Catalytic / metabolism
  • Structure-Activity Relationship

Substances

  • RNA, Catalytic
  • RNA