Fas-activated serine/threonine kinase (FAST) phosphorylates TIA-1 during Fas-mediated apoptosis

J Exp Med. 1995 Sep 1;182(3):865-74. doi: 10.1084/jem.182.3.865.


We have identified a serine/threonine kinase that is rapidly activated during Fas-mediated apoptosis. Fas-activated serine/threonine kinase (FAST) is phosphorylated on serine and threonine residues in Jurkat cells. In response to Fas ligation, it is rapidly dephosphorylated and concomitantly activated to phosphorylate TIA-1, a nuclear RNA-binding protein that has been implicated as an effector of apoptosis. Phosphorylation of TIA-1 precedes the onset of DNA fragmentation, suggesting a role in signaling downstream events in the apoptotic program. Our results introduce Fast and TIA-1 as components of a molecular cascade involved in signaling Fas-mediated apoptosis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Antigens, Surface / physiology*
  • Apoptosis / physiology*
  • Consensus Sequence
  • DNA, Complementary / genetics
  • Humans
  • Leukemia-Lymphoma, Adult T-Cell
  • Membrane Proteins*
  • Molecular Sequence Data
  • Phosphorylation
  • Poly(A)-Binding Proteins
  • Protein Processing, Post-Translational*
  • Protein Serine-Threonine Kinases / isolation & purification
  • Protein Serine-Threonine Kinases / metabolism*
  • Proteins*
  • RNA-Binding Proteins / metabolism*
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • T-Cell Intracellular Antigen-1
  • Tumor Cells, Cultured
  • fas Receptor


  • Antigens, Surface
  • DNA, Complementary
  • Membrane Proteins
  • Poly(A)-Binding Proteins
  • Proteins
  • RNA-Binding Proteins
  • T-Cell Intracellular Antigen-1
  • TIA1 protein, human
  • fas Receptor
  • FASTK protein, human
  • Protein Serine-Threonine Kinases

Associated data

  • GENBANK/X86779