A new method of human cardiac troponin I and troponin T purification

Biochem Mol Biol Int. 1995 May;36(1):195-202.


New rapid and effective procedure for simultaneous purification of human cardiac troponin I and troponin T has been developed. Affinity chromatography on immobilized monoclonal antitroponin I antibody C5 was used for purification of the whole troponin complex with a yield of 120 mg from 100 g of tissue. Isolated troponin I and troponin T (about 20 and 35 mg from 100 g tissue) were obtained by conventionally used ion-exchange chromatography. Antibody C5 recognizes conservative epitope of troponin I, therefore the method is applicable for purification of skeletal and cardiac troponin from a number of different animal species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Blotting, Western
  • Chromatography, Affinity / methods
  • Chromatography, Ion Exchange / methods
  • Epitopes / immunology
  • Humans
  • Myocardium / chemistry*
  • Troponin / immunology
  • Troponin / isolation & purification*
  • Troponin I
  • Troponin T


  • Antibodies, Monoclonal
  • Epitopes
  • Troponin
  • Troponin I
  • Troponin T