Identification of murine loci associated with susceptibility to chronic experimental autoimmune encephalomyelitis

Nat Genet. 1995 Jul;10(3):313-7. doi: 10.1038/ng0795-313.


B10.RIII mice develop chronic and relapsing experimental autoimmune encephalomyelitis (EAE) after immunization with the myelin basic protein (MBP) peptide 89-101. The disease is associated with the major histocompatibility complex (MHC) (eae1). We have now investigated the importance of non-MHC regions for the EAE susceptibility in a cross between RIIIS/J and B10.RIII mice which share the MHC region but differ in disease susceptibility. Linkage analysis using microsatellite markers spanning the genome identified a region (eae2) on chromosome 15 which showed linkage to disease (P = 0.0002). Our data also suggest linkage to a second region (eae3) on chromosome 3 (P = 0.0024), and provide evidence for locus interactions between eae2 and eae3. These results provide clues to the genetic basis of multiple sclerosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Chromosome Mapping
  • Crosses, Genetic
  • DNA, Satellite / genetics
  • Encephalomyelitis, Autoimmune, Experimental / etiology
  • Encephalomyelitis, Autoimmune, Experimental / genetics*
  • Female
  • Genetic Linkage
  • Genetic Markers
  • Genotype
  • Immunization
  • Male
  • Mice
  • Molecular Sequence Data
  • Myelin Basic Protein / genetics
  • Myelin Basic Protein / immunology
  • Peptide Fragments / genetics
  • Peptide Fragments / immunology
  • Phenotype
  • Species Specificity


  • DNA, Satellite
  • Genetic Markers
  • Myelin Basic Protein
  • Peptide Fragments