Lipofection reagents prepared by a simple ethanol injection technique

Biotechniques. 1995 Jun;18(6):1027-32.


Cationic liposomes are being utilized for the delivery of DNA into mammalian cells both in vitro and in vivo. In this report, we describe a rapid, simple injection method for the preparation of cationic liposomes that requires no special equipment. We demonstrate that, using this injection method, liposomes prepared with a commercially available cationic lipid (dimethyldioctadecylammonium bromide, DDAB) or a synthetic cationic cholesterol derivative, along with a neutral lipid (dioleoyl-L-alpha-phosphatidylethanolamine, DOPE), effectively transfect a variety of cell lines in vitro. The transfection activity of liposomes prepared by this method was comparable to that obtained with liposomes prepared by the standard evaporation/sonication procedure and to that of a commercial reagent, Lipofectamine.

Publication types

  • Research Support, Non-U.S. Gov't
  • Technical Report

MeSH terms

  • Cations
  • Cell Line / metabolism
  • DNA / metabolism
  • Electrophoresis, Agar Gel
  • Ethanol*
  • Indicators and Reagents / pharmacology*
  • Liposomes*
  • Microinjections / methods*
  • Phosphatidylethanolamines
  • Quaternary Ammonium Compounds
  • Transfection / methods*


  • Cations
  • Indicators and Reagents
  • Liposomes
  • Phosphatidylethanolamines
  • Quaternary Ammonium Compounds
  • didodecyldimethylammonium
  • Ethanol
  • 1,2-dielaidoylphosphatidylethanolamine
  • DNA