Synergistic regulation of a neuronal chloride current by intracellular calcium and muscarinic receptor activation: a role for protein kinase C

Neuron. 1995 Sep;15(3):729-37. doi: 10.1016/0896-6273(95)90160-4.


Using perforated patch recordings in combination with intracellular Ca2+ ([Ca2+]i) fluorescence measurements, we have identified a delayed Ca(2+)-dependent Cl- current in a mammalian sympathetic ganglion cell. This Cl- current is induced by the synergistic action of Ca2+ and diacylglycerol (DAG) and is blocked by inhibitors of protein kinase C. As a result, the current can be induced by acetylcholine through the conjoint activation of nicotinic receptors (to produce a rise in [Ca2+]i) and muscarinic receptors (to generate DAG). This demonstrates an unusual form of synergism between the two effects of a single transmitter mediated via separate receptors operating within a time scale that could be of physiological significance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcholine / pharmacology
  • Animals
  • Calcium / metabolism*
  • Calcium / pharmacology
  • Chloride Channels / physiology*
  • Diglycerides / pharmacology
  • Drug Synergism
  • Electric Conductivity
  • Ganglia, Sympathetic / cytology
  • Neurons / physiology*
  • Protein Kinase C / antagonists & inhibitors
  • Protein Kinase C / metabolism*
  • Rats
  • Receptors, Muscarinic / drug effects
  • Receptors, Muscarinic / physiology*
  • Receptors, Nicotinic / drug effects
  • Receptors, Nicotinic / physiology


  • Chloride Channels
  • Diglycerides
  • Receptors, Muscarinic
  • Receptors, Nicotinic
  • Protein Kinase C
  • Acetylcholine
  • Calcium